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THETM His Tag Antibody [HRP], mAb, Mouse

Catalog No. Size Price Figures
A00612-100 ug
 		100 ug
$ 410.00
MSDS:  20080213022807  (PDF)
MSDS:  20090114012119  (PDF)
COA:  A00612_142907_1_COA  (PDF)
COA:  A00612_11K000403  (PDF)

EXAMPLE:
Mouse Anti His-tag [HRP] mAb
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Full Name
THETM His Tag Antibody [HRP], mAb, Mouse
AliasHis-tag Antibody [HRP],mAb, Mouse
DescriptionGenScript THETM His Tag Antibody [HRP], mAb, Mouse is HRP conjugated THETM Anti-His Monoclonal Antibody (Mouse) (A00186) purified from mice ascites by protein A affinity column. This product is suitable for detecting the expression level of His fusion proteins or His by various immunoassays.
Ig SubclassIgG1, k
StorageThe antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
SpecificityTHETM His Tag Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Concentration0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal
Clone6G2A9
ImmunogenA synthetic peptide HHHHHH coupled to KLH
Purification MethodProtein A affinity column
ReconstitutionReconstitute the lyophilized antibody with deionized water to the final concentration of 0.5 mg/ml.
ApplicationsWorking concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
ConjugationHorseradish Peroxidase (HRP)
Host SpeciesMouse
NoteBriefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical ManualTM0589, TM0590, TM0591, TM0592, TM0593
* For Non-Clinical Research Use Only *
Related Products:
CodeNameSizePrice 
A00174antibody : His-tag Antibody, pAb, Rabbit40 ug
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200 ug
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A00186 antibody : THETM His Tag Antibody, mAb, Mouse40 ug
$ 180.00
100 ug
$ 375.00
200 ug
$ 675.00
A00613antibody : THETM His Tag Antibody [Biotin], mAb, Mouse100 ug
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